The comparative enzymology and cell origin of rat hepatomas. IV. Pyrimidine metabolism in minimal-deviation tumors.
نویسندگان
چکیده
rived from the primary tumors induced with ethionine. Normal liver, Dunning L-C18 hepatoma, and Morris 5123 hepatoma were similar in having almost no deoxycytidylate deaminase, whereas the Novikoff hepatoma was shown to be a rich source of the enzyme, converting 400-600 ¿¿moles substrate/gm protein/hr. Other hepatomas had values between 50 and 500 /umoles/gm protein/hr. The Morris 5123 hepatoma contained "thymine reducÃ-ase"with a capacity of 9-23
منابع مشابه
The Comparative Enzymology and Cell Origin of Rat Hepatomas
Received for publication August 16, 1965. to parenchymal liver cells in both morphology (18, 19) and enzyme pattern (@1, @6). This hepa toma has been called a “minimal-deviation― tu mor in contradistinction to multiple-deviation tu mors which may differ from their normal pro genitor so much as to make the identification of the cell of origin difficult if not impossible (@5). In addition, th...
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متن کاملThe comparative enzymology and cell origin of rat hepatomas. I. Deoxycytidylate deaminase and thymine degradation.
Enzyme assays for deoxycytidylate deaminase and thymine catabolism were carried out on ten rat hepatomas, including the Novikoff, Dunning L-C18, McCoy MDAB, and the Morris 3683, 3924A, and 5128 hepatomas, plus primary hepatomas produced by feeding ethionine or 8'-methyl-4-dimethylaminoazobenzene, and hepatomas derived from the primary tumors induced with ethionine. Normal liver, Dunning L-C18 h...
متن کاملThe comparative enzymology and cell origin of rat hepatomas. II. Glutamate dehydrogenase, choline oxidase, and glucose-6-phosphatase.
The activities of glucose-6-phosphatase, glutamate dehydrogcnase, and choline ox[dase were determined in some or all of ten rat hepatomas, including the Novikoff, Dunning L-C18, McCoy MDAB, and the Morris 3683, 39524A, and 51~3 hepatomas, together with primary hepatomas produced by feeding ethionine or 3%nethyl-4dimethylaminoazobenzene, and transplanted hepatomas derived from the primary tumors...
متن کاملPyrimidine Metabolism in Tissue Culture Cells Derived from Rat Hepatomas I. Suspension Cell Cultures Derived from the Novikoff Hepatoma'
hepatomas have demonstrated that a spectrum of such tumors exists with varying degrees of change from the parent hepatocyte (26—30). Such hepatocarcinomas as the Morris 5123 (18) and Reuber H-35 (34), for instance, resemble the parent tissue to a marked degree both morphologically and biochemically (27), and thus fall in the category termed “minimaldeviation hepatomas― by Potter (31). On ...
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عنوان ژورنال:
- Cancer research
دوره 23 شماره
صفحات -
تاریخ انتشار 1963